FAQ  •  Register  •  Login

Dialysis to remove glycerol before metal labeling

Forum rules
It is fine to promote your company's reagents. Just make sure they are relevant to CyTOF, and do so in moderation and style :-)
<<

esimonin

Participant

Posts: 1

Joined: Fri Oct 20, 2023 8:42 pm

Post Thu Dec 14, 2023 7:41 pm

Dialysis to remove glycerol before metal labeling

Hello, I am labelling antibodies with Maxpar X8 polymer. Some of my antibodies come in 30-50% glycerol (otherwise they are BSA/carrier free). I have previously dialyzed glycerol-containing antibodies overnight into PBS using slide-a-lyzer cassettes. I am wondering for anyone who has dialyzed first, if I need to dialyze overnight or if 10-60 min is sufficient? I see on other posts discussion that the glycerol is removed in the first R-buffer exchange and dialysis might not even be necessary. Curious anyone's experience with this. Thank you!
<<

ghowell

Participant

Posts: 19

Joined: Wed Nov 26, 2014 3:18 pm

Post Thu Jan 04, 2024 11:49 pm

Re: Dialysis to remove glycerol before metal labeling

Hi Elisabeth, apologies for the slow posting of this reply.
I've done a few conjugations now with a spin column protocol which seems to work very nice for removing glycerol up to 50%, I've never dialysed. Happy to pass it on if you think it would be helpful? Gareth

Return to Reagents for CyTOF

Who is online

Users browsing this forum: No registered users and 0 guests

cron