Dialysis to remove glycerol before metal labeling
Hello, I am labelling antibodies with Maxpar X8 polymer. Some of my antibodies come in 30-50% glycerol (otherwise they are BSA/carrier free). I have previously dialyzed glycerol-containing antibodies overnight into PBS using slide-a-lyzer cassettes. I am wondering for anyone who has dialyzed first, if I need to dialyze overnight or if 10-60 min is sufficient? I see on other posts discussion that the glycerol is removed in the first R-buffer exchange and dialysis might not even be necessary. Curious anyone's experience with this. Thank you!