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Miltenyi REA antibodies conjugation

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It is fine to promote your company's reagents. Just make sure they are relevant to CyTOF, and do so in moderation and style :-)
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ChantelMcS

Participant

Posts: 8

Joined: Thu Sep 04, 2014 6:07 pm

Post Wed Jun 14, 2023 4:29 pm

Miltenyi REA antibodies conjugation

Hi! Do the Miltenyi REA antibodies behave well in metal conjugations? Using these antibodies might give a clearer signal which will help in our work on human cells.

Thank you! Chantel
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cguidos

Contributor

Posts: 28

Joined: Tue Nov 18, 2014 3:10 am

Post Wed Jun 14, 2023 5:23 pm

Re: Miltenyi REA antibodies conjugation

Hi Chantel - they work well for us!


Cynthia
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ChantelMcS

Participant

Posts: 8

Joined: Thu Sep 04, 2014 6:07 pm

Post Wed Jun 14, 2023 7:22 pm

Re: Miltenyi REA antibodies conjugation

Cynthia! Thank you! :-)
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nadjakuechler

Participant

Posts: 2

Joined: Mon Aug 26, 2024 11:27 am

Post Thu Aug 29, 2024 9:08 am

Re: Miltenyi REA antibodies conjugation

Hello Cynthia,

have you tried to couple an REA antibody with cadmium (MCP9, Fluidigm) or does your experience relate to lanthanides in particular?

Kind regards
Nadja
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mleipold

Guru

Posts: 6568

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Thu Aug 29, 2024 3:29 pm

Re: Miltenyi REA antibodies conjugation

Hi Nadja,

I have conjugated a few REA antibodies with X8/DN3, but I have not tried MCP9 conjugation with REA antibodies. However, since the actual conjugation chemistry is the same (TCEP-reduced antibody, maleimide-terminated polymer), they should work the same.

Have you tried REA+MCP9 and had problems?


I will say: like several other users, I have generally experienced slightly lower recovery with MCP9 (often 60%, based on Nanodrop A280) than with X8 (usually 70-80%). See discussion here: viewtopic.php?f=7&t=880&p=7352
- BTW, looks like Adeeb's 2019 post had a CD298 REA clone conjugation with MCP9.

Mike

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