Tue Jan 24, 2023 11:11 pm
103Rh in the MDIPA kits
Hello,
I would like to report that as a user of the Maxpar Direct Immune Profiling Assay (MDIPA) kits, I recently had some trouble with the Rh including in the lyophilized vials.
In my case, I stained thawed PBMCs as individual samples, then I fixed them (2% PFA in CyPBS) and permeabilized them with the 10X perm buffer included in the Pd barcoding kit from Standard Biotools. After barcoding, I pooled the cells and stained them with the Ir-intercalator.
With this sequence, I noticed that the rhodium staining is too weak to properly discriminate the dead cells from the live cells. This induces major background in some of the populations, resulting in unusable samples for analysis.
I reported the problem to STB and it is currently under investigation. I know that at least another group of collaborators has similar issues with these kits.
As a reference, I currently use several MDIPA kits from the lot #2202087-10-A, all of them have the same problem.
If you noticed something similar, don't hesitate to report the problem to STB. It might help them to improve the QC on the Rh and the MDIPA kits in general.
Regards,
Caroline
I would like to report that as a user of the Maxpar Direct Immune Profiling Assay (MDIPA) kits, I recently had some trouble with the Rh including in the lyophilized vials.
In my case, I stained thawed PBMCs as individual samples, then I fixed them (2% PFA in CyPBS) and permeabilized them with the 10X perm buffer included in the Pd barcoding kit from Standard Biotools. After barcoding, I pooled the cells and stained them with the Ir-intercalator.
With this sequence, I noticed that the rhodium staining is too weak to properly discriminate the dead cells from the live cells. This induces major background in some of the populations, resulting in unusable samples for analysis.
I reported the problem to STB and it is currently under investigation. I know that at least another group of collaborators has similar issues with these kits.
As a reference, I currently use several MDIPA kits from the lot #2202087-10-A, all of them have the same problem.
If you noticed something similar, don't hesitate to report the problem to STB. It might help them to improve the QC on the Rh and the MDIPA kits in general.
Regards,
Caroline
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