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Live- or Fixed-Cell Barcoding With TeMal 7 Isotopes

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TomerWeizmann

Contributor

Posts: 43

Joined: Mon Apr 07, 2014 11:58 am

Location: Weizmann Institute of Science - Israel

Post Fri Mar 01, 2024 10:50 am

Live- or Fixed-Cell Barcoding With TeMal 7 Isotopes

Standard BioTools released a new barcoding solution based on Tellurium isotopes.
Please see attached.
1- Suitable for fresh and fixed cells
2- Universal (any cell type and species)
3- 7 isotopes allows for the multiplexing of up to 35 samples in 7-choose-3 scheme (can combined to cisplatin isotopes for 126 plex).
Please share your input on this product.
Yours,
Tomer
Attachments
TeMal 7 isotopes bundle Flyer.pdf
(747.72 KiB) Downloaded 60 times
TeMal 7 Isotopes Technical Data Sheet.pdf
(123.6 KiB) Downloaded 54 times
TeMal 7 Isotopes FAQs (FLDM-01168 Rev 02) 202401.pdf
(293.45 KiB) Downloaded 42 times
Dr. Tomer-Meir Salame
Head, Mass Cytometry Unit
Life Sciences Core Facilities
Weizmann Institute of Science
E-mail: tomer-meir.salame@weizmann.ac.il
https://www.weizmann.ac.il/LS_CoreFacil ... etry/about
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mleipold

Guru

Posts: 5845

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Fri Mar 01, 2024 4:58 pm

Re: Live- or Fixed-Cell Barcoding With TeMal 7 Isotopes

Hi all,

FYI: this product is not listed yet on the SBio Storefront. However, you *can* order it, you'll probably just need to contact your FAS or Sales agent to get pricing info.


Background: these are the TeMal reagents first described from Mark NItz's lab: http://dx.doi.org/10.1002/cyto.a.23495

Chris Tape and others have been using it in collaboration with the Nitz Lab for a while, so there are papers for additional protocol hints in case the SBio documents are a bit sparse on details (for example, see TOBis section in Methods here: https://www.nature.com/articles/s41592-020-0737-8 ).


Researchers at UNC have also made variations on this using Selenium (SeMal) for other channels. That work hasn't been formally published yet, but has been in a couple posters at recent CYTOs. So, maybe this will expand further?


Mike

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