Miltenyi REA antibodies conjugation

[ChantelMcS]

Hi! Do the Miltenyi REA antibodies behave well in metal conjugations? Using these antibodies might give a clearer signal which will help in our work on human cells.

Thank you! Chantel

[cguidos]

Hi Chantel - they work well for us!


Cynthia

[ChantelMcS]

Cynthia! Thank you!

[nadjakuechler]

Hello Cynthia,

have you tried to couple an REA antibody with cadmium (MCP9, Fluidigm) or does your experience relate to lanthanides in particular?

Kind regards
Nadja

[mleipold]

Hi Nadja,

I have conjugated a few REA antibodies with X8/DN3, but I have not tried MCP9 conjugation with REA antibodies. However, since the actual conjugation chemistry is the same (TCEP-reduced antibody, maleimide-terminated polymer), they should work the same.

Have you tried REA+MCP9 and had problems?


I will say: like several other users, I have generally experienced slightly lower recovery with MCP9 (often 60%, based on Nanodrop A280) than with X8 (usually 70-80%). See discussion here: viewtopic.php?f=7&t=880&p=7352
- BTW, looks like Adeeb's 2019 post had a CD298 REA clone conjugation with MCP9.

Mike