CyTOForum

Hi Scott,

If the CyTOF has a maximum focus of 159-169, does that apply for both CyTOFv1 and CyTOFv2?

In other words, the expanded mass window of a CyTOFv2 (78-212 amu, according to your talk at CYTO 2013) would still peak at 159-169 like a CyTOF v1, even though (relative to a CyTOFv1) the mass window got extended slightly more to the Low mass end than the High mass end?


Thanks
Mike

Hi Mike,

Did you get any feedback to your question regarding the difference between CyTOF1 and 2 ?

Thank you,

Hugo

Hi Hugo,

No, I never got an official reply from DVS/Fluidigm regarding the 159-169 focus on CyTOFv1 vs CyTOFv2.

Hopefully the new DVS commentator will make an official post about it.


Thanks for the followup,
Mike

The CyTOF 2 and CyTOF both are set up to optimize the transmission of ions in the range 159-169 amu.
For more scientific details: For ions of similar ionization efficiency, the response (on a per atom basis, that is after correction for atomic weight) is principally determined by the transmission efficiency of the ion optics. The focusing of ions is mass dependent. And, a priori, the ion optics can be adjusted to favor certain mass ranges over others. The CyTOF 2 and CyTOF both are set up to optimize the transmission of ions in the range 159-169 amu.