HIMC Controls-LiveIntactSinglets now in FR
Posted: Mon Mar 13, 2023 5:47 pm
Hi all,
Several years ago, I uploaded the HIMC healthy PBMC controls dataset (FR-FCM-ZYAJ), which are Raw, Fluidigm-ver2-normd, and MATLAB-normd Ungated files of several healthy control cryoPBMC donors.
Since then, I've been asked a few times for LiveIntactSinglet files. Also, over the years, several people have misinterpreted the ZYAJ dataset as LiveIntactSinglet, rather than Ungated (ie, still containing debris, beads, dead cells, etc).
So, I have created a separate FR entry of the corresponding LiveIntactSinglets for Raw, Fluidigm-ver2-normd, and MATLAB-normd files: FR-FCM-Z64L
* these are the files that should be used for further clustering analysis, etc.
Have fun!
Mike
Several years ago, I uploaded the HIMC healthy PBMC controls dataset (FR-FCM-ZYAJ), which are Raw, Fluidigm-ver2-normd, and MATLAB-normd Ungated files of several healthy control cryoPBMC donors.
Since then, I've been asked a few times for LiveIntactSinglet files. Also, over the years, several people have misinterpreted the ZYAJ dataset as LiveIntactSinglet, rather than Ungated (ie, still containing debris, beads, dead cells, etc).
So, I have created a separate FR entry of the corresponding LiveIntactSinglets for Raw, Fluidigm-ver2-normd, and MATLAB-normd files: FR-FCM-Z64L
* these are the files that should be used for further clustering analysis, etc.
Have fun!
Mike