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Cytokine staining in IMC
Posted:
Tue Nov 10, 2020 5:08 pmby Paulan
Hi,
Has anybody tried to detect cytokines (besides IL1b) using IMC? I'm assuming it's difficult but wondering what people have tried and if anything has worked for them so far. Any indication that it does not work is also very welcome.
Thanks,
Paula
Re: Cytokine staining in IMC
Posted:
Tue Nov 10, 2020 10:41 pmby dianebender
Hi Paula,
Our group has imaged TNFa, IL-6, IFNg and IL-10 in human bone marrow or spleen using IMC. The IL-10 was a Fluidigm CyTOF antibody cat#3166008B used at 1:50 following the Fluidigm suggested AR protocol for FFPE. The other antibodies were not Fluidigm.
Diane
Re: Cytokine staining in IMC
Posted:
Wed Nov 11, 2020 10:27 amby Paulan
Hi Diane,
Thanks for your reply! Good to hear that it can be done. Would you say the cytokines detected were mostly cell associated/intracellular or did you also detect released cytokines in the tissue?
Cheers,
Paula
Re: Cytokine staining in IMC
Posted:
Wed Nov 11, 2020 5:44 pmby dianebender
Hi Paula,
The cytokines were cell associated/intracellular.
Diane
Re: Cytokine staining in IMC
Posted:
Thu Nov 12, 2020 12:05 pmby XMUCyTOF
Hi,
Do you need an inhibitor to stop the cytokine secreting from the intracellular?
Re: Cytokine staining in IMC
Posted:
Wed Nov 18, 2020 12:42 pmby Paulan
Hi Diane,
Thanks for your reply, sorry I forgot to check back but good to know they are intracellular/cell associated!
Cheers,
Paula