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strange tissue?

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nataliaCW

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Posts: 4

Joined: Mon Jan 31, 2022 4:42 pm

Post Mon May 29, 2023 8:27 am

strange tissue?

Hi everyone! I have just started my adventure with IMC. I need to stain a breast cancer OCT sample, but I am getting a very strange image (I let the section dry overnight). Does anyone have any idea what is going on with it?
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ReeceD

Participant

Posts: 2

Joined: Fri Jan 06, 2023 8:58 am

Post Wed Sep 13, 2023 8:17 am

Re: strange tissue?

Hi Natalia,

I have seen this a few times on different tissues. It is likely oil possibly from the triton step. You can try some extra washes with MQ Water that has usually worked for me!

Best wishes,
Reece

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