Exosome Detection

[TaReks]

Hello!

My team and I are looking to investigate exosomes using the CyTOF and looking for some input.

Exosomes are tricky when it comes to CyTOF. It is my understanding that there are issues mainly in the detection. The reason is that often exosomes or other small extracellular vesicles cannot actually “fit” enough antibody or cell-ID reagents on the exosome to be sensed by the detector. The limit of detection is around 350 antibodies per “cell.”

We have discussed a few methods including aggregation or physically expanding the exosomes to allow for more detection signal. However, this specific work is limited.

Any methods or publications out there that might help us?

Any support is greatly appreciated!

Thanks all!

[dianebender]

Hi Talis,
You could try using a new technology (not CyTOF) called SMCxPro by
MilliporeSigma. It is an immunoassay system (Erenna system) that can do
single molecule counting (SMC) if you have an antibody pair i.e. A capture
antibody and a detection antibody.

Diane

[mleipold]

Hi Talis,

Here's a publication that might help you get started: they looked at synaptosomes by CyTOF.
viewtopic.php?f=10&t=1252

[Fataneh]

hello
I want to ask the possibility of using CyTOF technology for detecting exosomes.

Would appreciated hearing your thought.

[mleipold]

In addition to the Synaptosome paper mentioned earlier in the thread, there have been a couple papers looking at exosomes:

https://doi.org/10.1016/j.biomaterials.2020.120152

https://doi.org/10.1039/D0NR02344B