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CyTOF for extracellular vesicles

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DevanjanDey

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Posts: 1

Joined: Fri Aug 23, 2024 8:58 pm

Post Mon Oct 21, 2024 3:02 pm

CyTOF for extracellular vesicles

Hi,

I would like to inquire if CyTOF technology has been used for the characterization of extracellular vesicles (EVs). If so, how can we effectively distinguish the EV population from debris? In the case of cells, we typically use an intercalator that binds to DNA for identification. Is there an alternative approach for distinguishing EVs?

Regards
Devanjan
University of Minnesota
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mleipold

Guru

Posts: 6548

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Oct 22, 2024 2:58 pm

Re: CyTOF for extracellular vesicles

Hi Devanjan,

A few people have done exosomes (or exosome-like), but like any kind of exosome or similar "particle", a lot can depend on the source of them (and therefore the content). And, total metal content is really important, to make sure they're detected properly (which was also the case for the organelle work at UMN: http://dx.doi.org/10.1021/acs.analchem.8b02790)

Here's an earlier thread to help get you started: viewtopic.php?f=1&t=1350&p=5475


Mike

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