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mleipold

Guru

Posts: 2095

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Jan 21, 2014 9:02 pm

Re: Basics questions

Hi Scott,

If the CyTOF has a maximum focus of 159-169, does that apply for both CyTOFv1 and CyTOFv2?

In other words, the expanded mass window of a CyTOFv2 (78-212 amu, according to your talk at CYTO 2013) would still peak at 159-169 like a CyTOF v1, even though (relative to a CyTOFv1) the mass window got extended slightly more to the Low mass end than the High mass end?


Thanks
Mike
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HugoTharinger

Participant

Posts: 6

Joined: Fri Nov 22, 2013 10:21 am

Post Tue May 27, 2014 11:59 pm

Re: Basics questions

Hi Mike,

Did you get any feedback to your question regarding the difference between CyTOF1 and 2 ?

Thank you,

Hugo
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mleipold

Guru

Posts: 2095

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Wed May 28, 2014 3:08 pm

Re: Basics questions

Hi Hugo,

No, I never got an official reply from DVS/Fluidigm regarding the 159-169 focus on CyTOFv1 vs CyTOFv2.

Hopefully the new DVS commentator will make an official post about it.


Thanks for the followup,
Mike
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anitamkant

Contributor

Posts: 46

Joined: Mon Nov 18, 2013 6:30 am

Post Thu May 29, 2014 1:32 am

Re: Basics questions

The CyTOF 2 and CyTOF both are set up to optimize the transmission of ions in the range 159-169 amu.
For more scientific details: For ions of similar ionization efficiency, the response (on a per atom basis, that is after correction for atomic weight) is principally determined by the transmission efficiency of the ion optics. The focusing of ions is mass dependent. And, a priori, the ion optics can be adjusted to favor certain mass ranges over others. The CyTOF 2 and CyTOF both are set up to optimize the transmission of ions in the range 159-169 amu.
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