Wed Feb 23, 2022 3:56 pm by MCOlivier
Hi Greg,
I used once (twice, see beelow) the expedeon Lightning-Link La139 labelling kit for a large panel with 42 antibodies.
My first attempt was a fail, as the first lot I recieved was unproperly shipped (we discovered it staied a while at room temp during shippment) and did not work. But the second attemp with a new, properly shipped lot, gave good labelling efficiency of the AB. After staining, the metal did not leak to other ABs as no significant background in 139 channel was observed.
To my knowledge, Fluidigm retrieved 139La from their catalog because the metal did not stay captured in their polymer, and, if remember correctly, the metal was uptaken in the other polymers of the panel, even if already coupled to metals. I do not know what kind of polymer is used in the expedeon kits, but it seems the the metal is retained properly.
Hope this helps !
Best
Olivier