FAQ  •  Register  •  Login

Bone marrow CD45 dim population

Forum rules
Please be as geeky as possible. Reference, reference, reference.
Also, please note that this is a mixed bag of math-gurus and mathematically challenged, so choose your words wisely :-)
<<

JoDFCI

Participant

Posts: 6

Joined: Thu Jul 11, 2019 6:38 pm

Post Thu Oct 21, 2021 12:20 pm

Bone marrow CD45 dim population

Need some bone marrow peeps opinion,

First time working on bone marrow
I am running healthy donor bm on cytof and have, using different donors and different panels (for now PBMC tailored panels unfortunately so not many bm markers), observed double CD45 pop. See the screenshot, gaussian cleaned singlets as pre-gating to this, doublets excluded.
Its also CD66b neg.
Is this to be expected?
Attachments
thumbnail_Screen Shot 2021-10-20 at 8.53.41 PM.png
thumbnail_Screen Shot 2021-10-20 at 8.53.41 PM.png (201.6 KiB) Viewed 2424 times
<<

taxkourel

Participant

Posts: 14

Joined: Sun Sep 03, 2017 3:46 pm

Post Mon Oct 25, 2021 4:20 pm

Re: Bone marrow CD45 dim population

yes this is quite common. I have not analyzed them separately TBH and always assumed these were less mature populations since this is BM.
<<

tomash

Contributor

Posts: 24

Joined: Sun Oct 19, 2014 10:15 pm

Post Mon Oct 25, 2021 10:26 pm

Re: Bone marrow CD45 dim population

Generally CD45 will progressively increase on cells as they differentiate away from the stem cells and into mature leukocytes (and progressively downregulate for erythrocytes etc). Final CD45 levels on depends on the lineage (e.g. low on neutrophils, high on lymphocytes etc).

You should see _some_ CD66b+ cells in that CD45low fraction, as CD66b comes up quite early on bone marrow neutrophils.

Return to CyTOF data analysis

Who is online

Users browsing this forum: No registered users and 3 guests