Smarttube and Maxpar Direct
Hello! I was reaching out to the CyTOF experts out there to get some advice with an issue. We had to collect blood samples from patients in a remote site with very little sample processing capabilities, so we decided to fix the whole blood with the Smarttube. Additionally, we have available in my lab some MAxparDirect kits that we would like to use with those samples. Unfortunately per this paper ( https://doi.org/10.1101/2020.06.26.20141341 ) looks like some of the clones in the kit are not capable of recognizing fixated antigens. Some of them of particular importance like CCR7. Did anybody encounter a way around this issue? I was thinking of trying other clones that might recognize fixated CCR7 and add them to the Maxpar mixture, but I am not sure if there are any. Any information would be greatly appreciated!!