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Cytokine staining in IMC

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Paulan

Participant

Posts: 6

Joined: Mon Aug 24, 2020 11:28 am

Post Tue Nov 10, 2020 5:08 pm

Cytokine staining in IMC

Hi,

Has anybody tried to detect cytokines (besides IL1b) using IMC? I'm assuming it's difficult but wondering what people have tried and if anything has worked for them so far. Any indication that it does not work is also very welcome.

Thanks,
Paula
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dianebender

Participant

Posts: 5

Joined: Mon Feb 11, 2019 1:21 am

Post Tue Nov 10, 2020 10:41 pm

Re: Cytokine staining in IMC

Hi Paula,
Our group has imaged TNFa, IL-6, IFNg and IL-10 in human bone marrow or spleen using IMC. The IL-10 was a Fluidigm CyTOF antibody cat#3166008B used at 1:50 following the Fluidigm suggested AR protocol for FFPE. The other antibodies were not Fluidigm.

Diane
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Paulan

Participant

Posts: 6

Joined: Mon Aug 24, 2020 11:28 am

Post Wed Nov 11, 2020 10:27 am

Re: Cytokine staining in IMC

Hi Diane,

Thanks for your reply! Good to hear that it can be done. Would you say the cytokines detected were mostly cell associated/intracellular or did you also detect released cytokines in the tissue?

Cheers,
Paula
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dianebender

Participant

Posts: 5

Joined: Mon Feb 11, 2019 1:21 am

Post Wed Nov 11, 2020 5:44 pm

Re: Cytokine staining in IMC

Hi Paula,
The cytokines were cell associated/intracellular.

Diane
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XMUCyTOF

Participant

Posts: 9

Joined: Tue Aug 07, 2018 3:09 am

Post Thu Nov 12, 2020 12:05 pm

Re: Cytokine staining in IMC

Hi,
Do you need an inhibitor to stop the cytokine secreting from the intracellular?
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Paulan

Participant

Posts: 6

Joined: Mon Aug 24, 2020 11:28 am

Post Wed Nov 18, 2020 12:42 pm

Re: Cytokine staining in IMC

Hi Diane,

Thanks for your reply, sorry I forgot to check back but good to know they are intracellular/cell associated!

Cheers,
Paula

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