conjugating monoclonal with gelatin present
many thanks
Toni
Cancer Research UK Manchester Institute (CRUK MI) The University of Manchester
Block 33 | Mereside Alderley Park| Alderley Edge| SK10 4TG |United Kingdom
http://www.cruk.manchester.ac.uk/
Contributor
Posts: 47
Joined: Mon Oct 05, 2015 9:48 am
Location: European Genomic Institute for Diabetes, Universitity of Lille, Institut Pasteur de Lille, France
MCOlivier wrote:Dear Tony,
It has been discussed previously in this forum, the solution for gelatin handling during conjugation being "simply" to increase centrifugations duration from 8 to 20 minutes :
viewtopic.php?f=1&t=1183&p=3473&hilit=gelatin#p3473
I tried and it worked well ^^
Regards
Olivier
Jahangir wrote:Hi Toni,
I've also had the same predicament in our lab and I found that increasing the centrifugation time does help.
However, with santa cruz, if you email them and ask them if they could supply the same antibody but without gelatin, I believe they can do that as that's what we've done in our lab whenever we want to purchase an antibody from santa cruz.
For example, with the GLI-1 antibody found on their page -> https://www.scbt.com/p/gli-1-antibody-c-1 , if you scroll down and look at the forms they can be purchased in. You'll see something called "GLI-1 Antibody (C-1) X". The 'X' stands for 'without gelatin'.
Try emailing them and hopefully they'll get back to you with a gelatin-free version of the antibody. Let us know how it goes!
Best,
Jahangir
Users browsing this forum: No registered users and 10 guests