Post Tue May 26, 2020 10:26 pm

2020-Catena et al-preprint

"Highly multiplexed molecular and cellular mapping of breast cancer tissue in three dimensions using mass tomography"
Raul Catena, Alaz Oezcan, Laura Kuett, Alex Pluess, Peter Schraml, Holger Moch, IMAXT Consortium, Bernd Bodenmiller
bioRxiv, posted May 26, 2020

- will update when/if this is peer-reviewed and published

- Data links at the bottom here: ... omography/
- though it looks like the 3D tsv dataset link is broken/missing

"Our MT approach is based on the serial sectioning of a punched tissue cylinder (1 mm diameter) from a paraffin block of archival tissue and subsequent 3D reconstruction from 2D IMC data acquired from each individual serial section (Fig. 1A). A major hurdle to achieve 3D tissue reconstruction is the generation of deformation-free sections from paraffin-embedded tissues. [..] After preparation of 156 consecutive slices from the same tissue, we stained the sections with a breast cancer-centric panel of antibodies."