Thu Oct 31, 2019 12:29 pm by DMcDonald
Hi All,
I did some limited testing of these about 18 months ago during a beta-test. I conjugated a huCD45 to 159Tb and had signal >10e3 duals. I haven't retested this Ab though recently which would be a nice to see the stability. Hopefully I'll have the chance to test some more of the kits now they're on the market as their product format for 10 ug reactions make them very amenable to many of the antibodies we get for IMC projects where you struggle to get 100 ug at an affordable price.
The two major benefits were recovery and time. As there are no spin steps then you can't really lose any Ab and it takes minutes not hours to perform a conjugation. Despite the superior recovery, they do have their pricing wrong though it should be brought more in line with Fluidigms pricing.
Looking forward to hearing how you get on as some healthy competition has been a long time coming in the field.
Best wishes,
David