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Pre-conditioning Cisplatin

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nkhanbham

Master

Posts: 53

Joined: Wed Feb 25, 2015 3:03 pm

Post Tue Aug 13, 2019 8:10 pm

Pre-conditioning Cisplatin

This is a follow up from the thread on "Getting monoisotopic cisplatin Pt195 and 196" viewtopic.php?f=7&t=1077&view=unread#unread

Can the 48hr at 37C cisplatin pre-conditioning step be performed upon receiving the vial from fluidigm and then aliquotted? Having to do it 48hr before every staining experiment could sometimes be inconvenient eg if staining on a Mon/Tue precondition would be on Sat/Sun.
NK
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mleipold

Guru

Posts: 2156

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Aug 13, 2019 8:15 pm

Re: Pre-conditioning Cisplatin

Hi Naeem,

From the Hartmann et al paper:
"Viability staining

Cisplatin (Sigma) was resuspended to 100mM in DMSO, pre-conditioned for 48 h at 37°C and stored at −20°C."


Therefore, unless Felix chimes in differently, I have to assume the cisplatin was conditioned *once*, upon dissolving, and then the conditioned cisplatin solution was stored at -20C for future use.


Mike
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nkhanbham

Master

Posts: 53

Joined: Wed Feb 25, 2015 3:03 pm

Post Tue Aug 13, 2019 8:24 pm

Re: Pre-conditioning Cisplatin

Your are right Mike - just looked at that paper.
Thanks Mike.

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