Fri Aug 16, 2019 6:08 am by JamesW
Hello all,
Just to chime in on this one previously I did 48 hours of preconditioning on DCED-palladium based on Felix's paper but this time I skipped it as my cisplatin got stuck in a shipping warehouse for several days and was pretty warm on arrival. I just aliquoted and put it in the Freezer.
At least my understanding of the "Say no to DMSO" paper linked in Mikes comment is that the observed loss of cytotoxicity must be pretty rapid as I think the authors just dissolve the cisplatin in DMSO right before use. Presumably, the cytotoxicity drops further with the suggested 48 hours of preconditioning but I don't have any information on how much.
Jahangir, I did just follow the Mei et al. protocol for the antibody labelling, it seems to have worked well.
Best,
James