CD66b lost in Frozen vs Fresh vs PBMCs
We would appreciate your insights on loss of CD66b+ populations in Frozen vs Fresh Human PBMCs prep after Ficoll.
The cells were frozen in FBS+10%DMSO.
We are familiar with the literature on the issue addressing MDSCs and their sensetivity to freezing, e.g.:
'Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis'
https://bmcimmunol.biomedcentral.com/ar ... LaWaItadxU
viSNE plot attached.
Any thoughts on how to preserve the cells as so not to lose them?
Many thanks!
Head, Mass Cytometry Unit
Life Sciences Core Facilities
Weizmann Institute of Science
E-mail: tomer-meir.salame@weizmann.ac.il
https://www.weizmann.ac.il/LS_CoreFacil ... etry/about