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FlowVS for transformation

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YWzhang

Participant

Posts: 2

Joined: Sat Mar 09, 2019 3:35 pm

Post Thu May 02, 2019 2:56 pm

FlowVS for transformation

Does anyone here tried FlowVS for cytof transformation? Dose it make difference? Any experience to share?
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bc2zbUVA

Contributor

Posts: 22

Joined: Thu Nov 19, 2015 4:23 pm

Post Tue May 07, 2019 5:36 pm

Re: FlowVS for transformation

I played around with it quite a bit. I presume you are referring to the R package? My biggest complaint is that it is (or at least was) not designed for use with CyTOF data, only flow. I found that by changing the code to test coefficient values around 5, it worked much better.
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YWzhang

Participant

Posts: 2

Joined: Sat Mar 09, 2019 3:35 pm

Post Mon May 13, 2019 4:06 pm

Re: FlowVS for transformation

Thanks for sharing. Yes, it is R package and I am planning to use it for Cytof. I tried to play with it but keep getting "Error in optimize(afun, b, maximum = TRUE) : 'xmin' not less than 'xmax'.

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