cisplatin 198 labelling
Regards,
NK
Khantain wrote:Hi,
I recently used 198Pt from Fluidigm with the protocol from Mei et al. and I got good results with an anti-CD66 antibody (I coupled 100ug of the TET2 clone). I tested this conjugation with the "compensation protocol" from Chevrier et al., 2018 (I recorded beads one by one, not pooled) and I found this 198Pt solution has relatively good purity but contains some 194, 195 and 196 traces. I join a screenshot of the "matrix compensation" generated by CATALYST. The spillover of 198 into the 194 channel is under 1.5% in my panel (CD66 into CD20).
If you are interested I can email you more plots of the stainings with 194 and 198Pt.
Regards,
Quentin
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