Low event counts
I am having a peculiar problem on the Helios which I am struggling to solve.
The event rate has suddenly fallen to extremely low levels- it hovers around 20 events/sec !! The flow rate is steady and the nebulizer does not have any clogs (checked under microscope). There is no loss of vacuum on the instrument either. I did find a cracked sample delivery line (silica) which I thought was the issue, but replacing it did not solve the issue either. I have changed every part I can think of, to no avail.
Tuning is okay and the bead test runs fine, but the cells are definitely disappearing somewhere! Has anyone encountered this issue previously? Any ideas how to resolve it?
Thank you in advance,
Santhosh