Re: Where did my cells go???
I was using a CyTOF2, software version 6.0.449.
I updated and tried various concatenation programs with the same two files so I can give you the most up to date info.
Concatenating with FlowJo v7 gives those display issues, not sure if v9 is the same because I'm on PC.
Concatenation done in the newest beta version of FlowJo vX looks fine, although the event length seems stretched when I loaded the file into some programs.
The Cytobank tool and the new concatenation tool in the DVS software (v626) also generated .fcs that matched the original files. They also both preserved the Time data for the individual samples, which was nice. The CyTOF software tool took longer to concatenate, though, and the resulting file was much bigger (196MB) than the files generated with the other programs (all around 70MB). The original .fcs added up to about 200MB so most of the programs actually compressed the files some.
The Cytobank tool can also export the file number as a parameter so you can gate the samples individually, FlowJo v10 sort of does this.
All but the file generated in FlowJo v7 looked great in Cytobank.
So for now, I still like the cytobank tool the best although the others (minus FlowJo 7) seemed okay.
If you want to see that problem file from v7 I can upload it
-Rob