Tue Feb 13, 2018 7:10 pm by GregBehbehani
Hi Jim,
Thanks for the information. I'll double-check the details from my technicians, but we usually just dissolve the chloride we get from Sigma in L-buffer at 1M (or the highest concentration we can achieve) we then dilute further to make a working stock. Occasionally, we have to add a bit of acid (usually HCL or HNO3 to get the metal to dissolve), but I don't think we needed to do this for 89Y. We keep our metals in the refrigerator (a hold-over from what we did in the Nolan Lab).
I believe the L-Buffer is important, since you want to be sure the metal solution stays acidic, but I otherwise can't think of a reason that things wouldn't have worked for you.
We'll go back and re-test our 89Y and 139La antibodies to see if we're starting to see any subtle loss of signal, but they definitely still work as of a few weeks ago.
I'll post some follow-up once we finish our tests.
best,
Greg