Difficulty with Nolan stand-alone debarcoder
I've been trying to use the Nolan stand-alone debarcoder on a Helios file (I'm not using the Fluidigm BC kit), and I haven't been able to get to the debarcoding part.
SingleCellDebarcoderR2013b_MacOSX, version 1.
https://github.com/nolanlab/single-cell ... -FCS-Files
I created my CSV key of Masses in columns, BC combinations in rows. I made sure to not leave the upper left cell blank. I can open the debarcoder, select my CSV key, and select my FCS file, but I then get nothing but a pop-up saying "Check your barcode key". It does not progress further.
1. Since raw Helios files output with *.FCS and some other software has had problems, I tried changing the filename to *.fcs. No effect.
2. I tried doing the "Paste special"/"Values" recommendation in case somehow Excel was messing that up. No effect.
3. The example CSV key has "102" as a BC channel, but just in case there was a parameter naming issue between CyTOF2 and Helios, I also tried "Pd102", since that's what FlowJo lists as the metal/mass. No effect, nor any of the other CSV variations that I tried.
Cytoforum is telling me that "The extension csv is not allowed." for attached files, so the relevant FCS, Xlsx, and CSV files can be found at: https://drive.google.com/drive/folders/ ... sp=sharing
I have to assume that it's something really trivial.......
Thanks,
Mike