Unusual staining in 140-150 range
Has anyone experienced the following unusual staining? We are getting events that are Iridium +ve (and Cisplatin -ve) but with intermediate levels of 140Ce signal (see attachment). These "unknown" events show very high positivity across 140-150 range, which includes markers that cannot all be co-expressed such as CD19, CD4, CD11b, CD34. The events are not EQ beads stuck to cells, as we still see the abnormal staining in samples run without beads added. In the example shown it is only 0.2% but we see upto 5% in some samples. This has been observed using blood and bone marrow and using three different independent antibody panels.
I welcome any feedback.
Thanks,
Naeem