Fri Feb 23, 2018 4:12 pm by mleipold
Hi Olivier,
I haven't tried loading MAXPAR with Rh103. A couple years ago, Henrik and I tried loading Rh103 into the maleimide-DOTA (I use it as a live-dead, though Bodenmiller et al used it as a BC reagent). We used Sigma #450286, as the high-purity chloride salt, and dissolved into L-buffer.
At best, we got mixed results. We compared it to our regular In115-mDOTA, and in some cases, it compared well, but in other preps, it didn't (had no signal on the same cells that In115 was showing signal).
Granted, DTPA (on the MAXPAR) and DOTA are different chelators, but they usually bind similarly. If you do want to try it, I'd suggest a lot of validations (including stability testing; just because you get it in there doesn't always mean it will stay in under staining conditions).
I think Henrik tried again after he went back to Germany; maybe he'll comment further?
Mike