cysteines used in antibody conjugation
Does anybody know whether the cysteine groups used to bind the polymer in MaxPar conjugation are restricted to the heavy chain or also include ones on the light chain? There are internal disulfide bonds on both heavy and light chains and potentially both could be used. On all the diagrams I have seen the polymer is always bound to the heavy chain.
Is it correct that these are more likely to be reduced by TCEP while the ones in the hinge region connecting the two heavy chains are both (or at least one is) intact?
Thanks,
Naeem
(This may be a topiuc previously discussed - if so please redirect me to the thread)
Is it correct that these are more likely to be reduced by TCEP while the ones in the hinge region connecting the two heavy chains are both (or at least one is) intact?
Thanks,
Naeem
(This may be a topiuc previously discussed - if so please redirect me to the thread)