140Ce??
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Re: 140Ce??
We ordered that isotope custom from Trace. I don't know of any non-custom source.
(By the way, that channel is fairly susceptible to high background. If you use it, a bright marker is a good idea.)
(By the way, that channel is fairly susceptible to high background. If you use it, a bright marker is a good idea.)
Re: 140Ce??
Hi,
I think you wouldn't be able to find one as the 140Ce channel is reserved exclusively for normalization beads. You could still do it, but you'll probably have to do your own conjugation.
Best,
Ratnadeep
I think you wouldn't be able to find one as the 140Ce channel is reserved exclusively for normalization beads. You could still do it, but you'll probably have to do your own conjugation.
Best,
Ratnadeep
Re: 140Ce??
Hi Jahangir,
You'd have to order the purified isotope from somewhere like Trace.
That being said, I'd be cautious in using Ce140 for antibody labeling, since we've seen several instances of high background in that channel (particularly with tissue-derived samples) that would preclude resolution of any meaningful staining distribution.
Adeeb
edit: oops looks like Zach already got this covered.
You'd have to order the purified isotope from somewhere like Trace.
That being said, I'd be cautious in using Ce140 for antibody labeling, since we've seen several instances of high background in that channel (particularly with tissue-derived samples) that would preclude resolution of any meaningful staining distribution.
Adeeb
edit: oops looks like Zach already got this covered.
Adeeb Rahman
Icahn School of Medicine at Mount Sinai, NYC
Icahn School of Medicine at Mount Sinai, NYC
Re: 140Ce??
Hi Adeeb and Zach,
Many thanks for your input, I greatly appreciate it! Just out of curiosity, why does this channel produce a lot of background noise? Is the isotope relatively unstable? Or does the Helios machine not pick up this channel that great?
Furthermore, there have been issues with Barium contamination which is situated at the 139 channel, do you think there is some sort of leak over into the 140 channel??
Many thanks again,
Jahangir
Many thanks for your input, I greatly appreciate it! Just out of curiosity, why does this channel produce a lot of background noise? Is the isotope relatively unstable? Or does the Helios machine not pick up this channel that great?
Furthermore, there have been issues with Barium contamination which is situated at the 139 channel, do you think there is some sort of leak over into the 140 channel??
Many thanks again,
Jahangir
Re: 140Ce??
Random question, why do you need Ce140?
(Something specific about that channel, or "just another" parameter?)
(Something specific about that channel, or "just another" parameter?)
Re: 140Ce??
just another parameter, literally nothing special about it at all lol
Re: 140Ce??
Fair enough
Re: 140Ce??
Regarding the source of the contamination, you might find this interesting if you haven't seen it:
"High-Dimensional Single Cell Mapping of Cerium Distribution in the Lung Immune Microenvironment of an Active Smoker"
Rahman AH, Lavin Y, Kobayashi S, Leader A, Merad M
Cytometry B Clin Cytom. 2017
http://dx.doi.org/10.1002/cyto.b.21545
"High-Dimensional Single Cell Mapping of Cerium Distribution in the Lung Immune Microenvironment of an Active Smoker"
Rahman AH, Lavin Y, Kobayashi S, Leader A, Merad M
Cytometry B Clin Cytom. 2017
http://dx.doi.org/10.1002/cyto.b.21545
Adeeb Rahman
Icahn School of Medicine at Mount Sinai, NYC
Icahn School of Medicine at Mount Sinai, NYC
Re: 140Ce??
Hi Jahangir,
You said: "Furthermore, there have been issues with Barium contamination which is situated at the 139 channel, do you think there is some sort of leak over into the 140 channel??"
Cerium is the *only* element with a 140 mass (at least naturally-occurring). In your example with Ba, there's no Ba139 isotope (only 130-138, with Ba138 as 72% of total Ba). However, in extreme situation, you can get a case of the "right leg" of the ion peak spilling into the TOF acquisition window of the M+1, regardless of whether Nature has an isotope there. In this situation, your signal/contamination at Ba138 would usually need to be screaming bright (probably >2000-3000 Dual; often a streak/tire track) in order to get "right leg" spill.
So, yes, in theory, a really screaming bright La139 could spill into the 140 channel.....in fact, I've seen this happen once and can show the plots if anyone's curious.
But I've never seen a Ba138 contamination so bad that it spilled into 140 channel (ie, M+2). If that happened, your 139 (M+1) results would be completely uninterpretable.
Mike
You said: "Furthermore, there have been issues with Barium contamination which is situated at the 139 channel, do you think there is some sort of leak over into the 140 channel??"
Cerium is the *only* element with a 140 mass (at least naturally-occurring). In your example with Ba, there's no Ba139 isotope (only 130-138, with Ba138 as 72% of total Ba). However, in extreme situation, you can get a case of the "right leg" of the ion peak spilling into the TOF acquisition window of the M+1, regardless of whether Nature has an isotope there. In this situation, your signal/contamination at Ba138 would usually need to be screaming bright (probably >2000-3000 Dual; often a streak/tire track) in order to get "right leg" spill.
So, yes, in theory, a really screaming bright La139 could spill into the 140 channel.....in fact, I've seen this happen once and can show the plots if anyone's curious.
But I've never seen a Ba138 contamination so bad that it spilled into 140 channel (ie, M+2). If that happened, your 139 (M+1) results would be completely uninterpretable.
Mike
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