Custom barcoding reagents
Hi all,
We would like develop our own barcoding reagents as we have found the barcoding kit from fluidigm unsuitable for our needs. We would like to use the CD45 based approach - theres some valuable info in this earlier thread viewtopic.php?f=10&t=262 but maybe things have moved on a little since then with the introduction of the HELIOS and new metal conjugates.
I was wondering if people could share any experience of their own barcoding approach and maybe help us to decide how we go about this. What would the best system need to include - would modelling it on fluidigms triple barcode be the best approach? What are the best mass channels to use for the CD45 barcodes? Is M+16 signal always going to be an issue if we decide to use mass channels within our staining panel range?
Any help much appreciated!
David.
We would like develop our own barcoding reagents as we have found the barcoding kit from fluidigm unsuitable for our needs. We would like to use the CD45 based approach - theres some valuable info in this earlier thread viewtopic.php?f=10&t=262 but maybe things have moved on a little since then with the introduction of the HELIOS and new metal conjugates.
I was wondering if people could share any experience of their own barcoding approach and maybe help us to decide how we go about this. What would the best system need to include - would modelling it on fluidigms triple barcode be the best approach? What are the best mass channels to use for the CD45 barcodes? Is M+16 signal always going to be an issue if we decide to use mass channels within our staining panel range?
Any help much appreciated!
David.