best Annexin V reagent?
Hi,
We are interested in dropping Annexin V into one of our T cell panels and I wanted to know what others experience was for this regarding best reagent for detection. It seems that the options are I conjugate a metal directly to the purified, or stain with a fluorescent antibody and get an anti-fluorochrome antibody (Annexin V not a Fluidigm catalog reagent). Can anyone offer any advice as to which reagents have worked the best or given the clearest signal? Cells in apoptosis are presumably a very small amount, so it seems that a decent amount of sensitivity would be needed. I Have essentially 2 channels that are open in my current panel to put Annexin V in (157Gd, 173Tb; I want to avoid some of the super low or high atomic mass isotopes). 157Gd seems like a good choice at first glance.
Greg Hopkins
We are interested in dropping Annexin V into one of our T cell panels and I wanted to know what others experience was for this regarding best reagent for detection. It seems that the options are I conjugate a metal directly to the purified, or stain with a fluorescent antibody and get an anti-fluorochrome antibody (Annexin V not a Fluidigm catalog reagent). Can anyone offer any advice as to which reagents have worked the best or given the clearest signal? Cells in apoptosis are presumably a very small amount, so it seems that a decent amount of sensitivity would be needed. I Have essentially 2 channels that are open in my current panel to put Annexin V in (157Gd, 173Tb; I want to avoid some of the super low or high atomic mass isotopes). 157Gd seems like a good choice at first glance.
Greg Hopkins