Ca2+ and Mg2+ commercial solutions?
Hi all,
I'm working on a customer development project, and one of the antibodies they want apparently requires Ca2+ and Mg2+ for function (binding to target epitope).
I typically use the Rockland PBS, which does not list Ca and Mg (though does not explicitly state that it is Ca-Mg-free). I also usually use 2mM EDTA in my antibody staining buffer; obviously I can't continue that.
Does someone have a recommendation for Ca2+ and/or Mg2+ commercial solutions that have been free of background for CyTOF?
Thanks,
Mike
I'm working on a customer development project, and one of the antibodies they want apparently requires Ca2+ and Mg2+ for function (binding to target epitope).
I typically use the Rockland PBS, which does not list Ca and Mg (though does not explicitly state that it is Ca-Mg-free). I also usually use 2mM EDTA in my antibody staining buffer; obviously I can't continue that.
Does someone have a recommendation for Ca2+ and/or Mg2+ commercial solutions that have been free of background for CyTOF?
Thanks,
Mike