Thu Nov 28, 2013 9:11 am
Maxpar kit compatability
Can the maxpar kit be used to couple metals to Fab'2 Ab fragments; or only complete Abs with intact heavy chains?
Maxpar kit compatability
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It is fine to promote your company's reagents. Just make sure they are relevant to CyTOF, and do so in moderation and style
It is fine to promote your company's reagents. Just make sure they are relevant to CyTOF, and do so in moderation and style
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Fri Dec 06, 2013 10:12 pm
Re: Maxpar kit compatability
The MAXPAR polymers use a maleimide to covalently link to free thiols: any species with a free thiol will react with it. So,
http://www.piercenet.com/method/antibod ... ulfhydryls
Fab'2 structure info: http://www.abcam.com/index.html?pagecon ... &rid=12722
Therefore, if I am understanding this struture correctly, hitting a Fab'2 with TCEP to generate free thiols would make it fall apart into single Fab' units. Those could potentially be conjugated by the polymer, but I doubt this is what you want.
Also, since you've removed most of the Fc region (and its thiols), you would probably get fewer polymers/antibody, and therefore lower metal signal.
http://www.piercenet.com/method/antibod ... ulfhydryls
Fab'2 structure info: http://www.abcam.com/index.html?pagecon ... &rid=12722
Therefore, if I am understanding this struture correctly, hitting a Fab'2 with TCEP to generate free thiols would make it fall apart into single Fab' units. Those could potentially be conjugated by the polymer, but I doubt this is what you want.
Also, since you've removed most of the Fc region (and its thiols), you would probably get fewer polymers/antibody, and therefore lower metal signal.
2 posts
• Page 1 of 1
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