MaxPar Antibody conjugation
1. How long would the antibody be stable in MaxPar CyTOF ready form (I have some purchased form Biolegend, which are described as partially reduced and not needing the buffer exchange steps in the Fluidigm labelling protocol) if stored at 4C? I have concerns that this may not be for long and one should try to conjugate as soon as possible.
2. When purifying the partially reduced antibody (after 37C incubation) is it add 300ul of C buffer to the antibody prep and then add this mixture to the 50kDa filter? Or is it add the antibody prep to the filter and then add the C buffer to the filter? The protocol isn't clear on this.
3. When washing the metal conjugated antibody there are repeat (total 4) washes with W buffer - by washes does the protocol mean to repeat the 10min centrifuge step and then wash?
Many thanks,
Naeem