Metals tubes
We got 9 months ago 20 tubes of metals containing each 20µl (good for 4 conjugation of 100µg).
We are not using the metal tube in one time, but in 4 different times, for 4 different antibodies.
For some of them, I recently realized I will not have enough volume to perform the fourth conjugation. Maybe there is a small evaporation, meaning that the metal get concentrated over the time (this could maybe explain a protein precipitation during conjugation? excess of metal is washed on the 3kDa amicon before combination with the antibody, but who knows?)
Yes I keep them at 4 degree, Yes I keep them cool when taking them out of the fridge, yes I centrifuge them before opening the tube.
I am wondering if you guys get the same problem sometimes and if it could be a good strategy to aliquot the metal (5µl in smaller tube) immediately after reception.
Thank you for your help
Cytofely
Mickael