Dynabeads clogging the tip of the injector?
I have been using Dynabeads (Human T-Activator CD3/CD28) to stimulate T cells and been running them through a Helios system. We were under the impression that they would be compatible with CyTOF. Unfortunately, we had clogging problems at the level of the injector with a build-up of suspicious reddy/brown coloured residue at the very tip. This residue is quite tough and difficult to remove from the tip. We are concerned that this might be due to Dynabeads. I am stimulating T cells at a 1:1 ratio, 1 x 106 T cells at a time, per sample.
Has anyone had any experience running Dynabeads through the CyTOF, specifically has anyone encountered this type of clogging?
Thanks,
Ben.