Maxpar conjugation kit
Hi everybody,
I am wondering if it happened to you to get a kind of precipitate in your final elution when doing a conjugation using Maxpar procedure? Nanodrop indicates there is no protein in my elution !
We perfectly follow the procedure, I checked every paramaters such as temperature, pH, concentration of the initial antibody, incubation time, etc... everything look OK
These purified antibodies have been already conjugated in different metals. The metals also have been used with success for others conjugations.
Polymer lot is still the same, and since we use 4 or 8 polymer tubes at the same time, we never have to store them outside the sealed bag.
We work at room temperature, and I decrease a little bit the time/speed with the centricon columns to make sure not to dry my protein (even if there is a safe volume at the bottom of the column).
I don't know at which step my prtoein/salts precipitate, I visualize it only at the final elution when I turn over the column.
Thank you very much for your help
Cytofely
Mickael
I am wondering if it happened to you to get a kind of precipitate in your final elution when doing a conjugation using Maxpar procedure? Nanodrop indicates there is no protein in my elution !
We perfectly follow the procedure, I checked every paramaters such as temperature, pH, concentration of the initial antibody, incubation time, etc... everything look OK
These purified antibodies have been already conjugated in different metals. The metals also have been used with success for others conjugations.
Polymer lot is still the same, and since we use 4 or 8 polymer tubes at the same time, we never have to store them outside the sealed bag.
We work at room temperature, and I decrease a little bit the time/speed with the centricon columns to make sure not to dry my protein (even if there is a safe volume at the bottom of the column).
I don't know at which step my prtoein/salts precipitate, I visualize it only at the final elution when I turn over the column.
Thank you very much for your help
Cytofely
Mickael