Eu beads from Bangs Laboratories
Hi all,
Recently, I saw an ad for Eu chelate beads from Bangs Laboratories: product# FC02F http://www.bangslabs.com/applications/t ... crospheres
-Also see attached PDS.pdf
They come in 3 sizes: 0.1um, 0.2um, and 0.3um; I'm not sure of the size of Fluidigm EQ beads, but I think EQ beads are bigger. You can buy 1mL, 5mL or 10mL.
1mL of 0.3um is around $150. The lot I got was supplied as 7e11 beads/mL; I had to do a serial dilution down to 1M-fold in order to get the concentration similar to undiluted EQ beads.
I ran undiluted EQ beads and 1M-fold diluted Bangs Eu beads on one of my Helios instruments. I did not compare with Fluidigm Eu-only beads. I have attached a PDF of FlowJo Eu151 vs Eu153 plots comparing the Bangs Eu beads vs the Fluidigm EQ beads. I also attached an Excel of Median, CV, and SD.
In short: the Bangs beads are dimmer than the EQ beads, but are still pretty respectable at >1e2 Dual. One advantage to the Bangs beads is that their surface has COOH groups; therefore, if you wanted to covalently attach something to the surface, you could.
-Mike
Recently, I saw an ad for Eu chelate beads from Bangs Laboratories: product# FC02F http://www.bangslabs.com/applications/t ... crospheres
-Also see attached PDS.pdf
They come in 3 sizes: 0.1um, 0.2um, and 0.3um; I'm not sure of the size of Fluidigm EQ beads, but I think EQ beads are bigger. You can buy 1mL, 5mL or 10mL.
1mL of 0.3um is around $150. The lot I got was supplied as 7e11 beads/mL; I had to do a serial dilution down to 1M-fold in order to get the concentration similar to undiluted EQ beads.
I ran undiluted EQ beads and 1M-fold diluted Bangs Eu beads on one of my Helios instruments. I did not compare with Fluidigm Eu-only beads. I have attached a PDF of FlowJo Eu151 vs Eu153 plots comparing the Bangs Eu beads vs the Fluidigm EQ beads. I also attached an Excel of Median, CV, and SD.
In short: the Bangs beads are dimmer than the EQ beads, but are still pretty respectable at >1e2 Dual. One advantage to the Bangs beads is that their surface has COOH groups; therefore, if you wanted to covalently attach something to the surface, you could.
-Mike