Post Fri Aug 12, 2016 2:02 pm

Staining with Anti-PE after fixing

Hi everyone,

I was wondering if anyone has tried to stain with the anti-PE of anti-APC secondary's that Fluidigm cells after fixing cells that were stained with the primary antibody? My concern is that the 1.6% PFA fixation might change the PE enough that it the secondary won't bind. I plan on running a small pilot to see today, but was curious if others have tried this method. I'm trying to shorten my incubation times before fixation as we're measuring pSTATs, so a 2-step protocol before fixing wouldn't work.

-Chad