Transcription Factor Buffer Kits
Hi All,
We have had some issues here with cell yeilds from using transcription factor buffer kit from Tonbo Bioscience. I'm not sure if it is a buffer kit issue or not, but wanted to reach out and ask what people prefered and whether they had similar issues with some kits, but not with others. Once we fix I generally centrifuge at 800xg for 5 min, so I dont feel like we're losing cells because of a lack of spinning. In some cases, though, we notice we are only getting 10-25% of our cells back. Any thoughts?
-Chad
We have had some issues here with cell yeilds from using transcription factor buffer kit from Tonbo Bioscience. I'm not sure if it is a buffer kit issue or not, but wanted to reach out and ask what people prefered and whether they had similar issues with some kits, but not with others. Once we fix I generally centrifuge at 800xg for 5 min, so I dont feel like we're losing cells because of a lack of spinning. In some cases, though, we notice we are only getting 10-25% of our cells back. Any thoughts?
-Chad