Re: Bismuth conjugation
Posted: Tue May 07, 2019 5:55 pm
Hello all,
I would like to resurrect this old post to get some new feedback.
Recently, we have had some issues with the 209Bi CD8 conjugates we have been getting done externally for us. It appears that either the titre we get is highly variable, or that the antibodies don't work well, or die off very quickly (the intensity of staining goes down and background creeps up to almost "swallow" the positive) I understand the challenges in the conjugation chemistry, so am certainly not saying this is unexpected.
We are looking to potentially start doing the 209Bi conjugations ourselves and wanted to get overall (updated) opinion on what the best source was for 209Bi and in what formats (Trace Sciences/Sigma, Chloride/Acetate?). I see some responses below but hope to get some updated opinions and sharing of experiences as mentioned.
If someone has a protocol too that is more specific to conjugating to Bi209, it would be very much appreciated to set us up for successful conjugations in the future.
Greg
I would like to resurrect this old post to get some new feedback.
Recently, we have had some issues with the 209Bi CD8 conjugates we have been getting done externally for us. It appears that either the titre we get is highly variable, or that the antibodies don't work well, or die off very quickly (the intensity of staining goes down and background creeps up to almost "swallow" the positive) I understand the challenges in the conjugation chemistry, so am certainly not saying this is unexpected.
We are looking to potentially start doing the 209Bi conjugations ourselves and wanted to get overall (updated) opinion on what the best source was for 209Bi and in what formats (Trace Sciences/Sigma, Chloride/Acetate?). I see some responses below but hope to get some updated opinions and sharing of experiences as mentioned.
If someone has a protocol too that is more specific to conjugating to Bi209, it would be very much appreciated to set us up for successful conjugations in the future.
Greg