Contributor
Posts: 44
Joined: Tue May 20, 2014 2:24 pm
Location: Linkoping University, Sweden and Finnish Institute of Molecular Medicine, Finalnd
Problem in finding Ly6D , CD127 and FLT+ population
we are looking for mouse Common Lymphoid Progenitor Compartment (CLP). The general gating strategy to find the CLP is: Lin-FLT3+CD127+ cKit+Sca-1+ intermediate> Ly6D+/-
Regarding antibody staining for Ly6D, CD127 and FLT3 looks fine. when we gate for Ly6D population from CLP we are getting 10% sometimes even less in CyTOF but the same experiment in flowcytometry gives around 60% population for Ly6D and it is acceptable. usually the Ly6D vs cKit population should be 40 :60%.
I think it is because of low staining or may be we need more events, I dont exactly.
any suggestion or help