Precipitation of polymer
Hi there,
I am having problems with precipitate forming when I dilute Palladium metals into L-buffer or amonium acetate (which is the same as L-buffer, I hear).
In the past I've used X8 polymer to load Palladium metals and conjugate to CD45, which has worked really well. However, I tried to repeat this and when I added the Palladium (which is in 5N HCl) to the L-buffer or to 20mM amonium acetate I get a very cloudy yellow solution which slowly starts to precipitate out. I had this once before when I used L-buffer, but not with home made amonium acetate, and could not explain it, and also cannot explain it now. Does anyone have an idea what's going on?
Advice is very welcome!
Dunja
I am having problems with precipitate forming when I dilute Palladium metals into L-buffer or amonium acetate (which is the same as L-buffer, I hear).
In the past I've used X8 polymer to load Palladium metals and conjugate to CD45, which has worked really well. However, I tried to repeat this and when I added the Palladium (which is in 5N HCl) to the L-buffer or to 20mM amonium acetate I get a very cloudy yellow solution which slowly starts to precipitate out. I had this once before when I used L-buffer, but not with home made amonium acetate, and could not explain it, and also cannot explain it now. Does anyone have an idea what's going on?
Advice is very welcome!
Dunja