Thu Sep 24, 2015 3:44 pm by CyTOFP4Hb
I'd like to label my antibodies with metal by using MaxPar Reagents. Two of my Ab are IgG which I can follow Nolan Lab's protocol. But one of my antibody is actually commercial whole goat antiserum which I bought from Sigma and it is already delipidized. My question is do I need to purify IgG from serum before metal labeling? Or I can label metal by using the serum (anyhow IgM can not be labeling based on the protocol) then purify metal-labeling IgG? Which way is more efficiency for labeling and less for losing my IgG?
I am new to CyTOF, any suggestion counts! Thank you so much in advance!!
Mei-Ling