Fri Jun 26, 2015 3:11 pm by mleipold
Hi Tina,
Try taking some of your 159Tb stock and diluting it ~1e5-1e6, and running it in solution mode like you would tuning solution. You should then be able to to see whether you still see 160-161.
You can also try staining BD Kappa capture beads with your antibodies of concern, and running them like cells.
Doing both would help you figure out whether the metal stock is contaminated, or your antibody stock, or whether it's coming from some other source (Ir, Pt, buffers, etc).
You can also buy trace-metal basis 99.9% pure TbCl3 from Sigma: #212903. You can make up a 50mM stock of that in L buffer, and use it for comparison....or as a substitute for the stock from Fluidigm.
Mike