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Polyclonal antibody conjugation

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DanaiBD

Participant

Posts: 7

Joined: Mon Oct 24, 2022 12:28 pm

Post Mon Oct 24, 2022 2:43 pm

Polyclonal antibody conjugation

Hello everyone. I have trouble finding a DIO2 antibody for my panel and I was wondering whether anyone has experience with conjugating polyclonals. I found an old post but hoping that more people may have tried it now. I am unsure what I should pay attention to.
The antibody I am looking at is: https://www.thermofisher.com/antibody/p ... /PA5-49631 in case that helps.
Thank you in advance for your help :D
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mleipold

Guru

Posts: 5442

Joined: Fri Nov 01, 2013 5:30 pm

Location: Stanford HIMC, CA, USA

Post Tue Oct 25, 2022 2:47 pm

Re: Polyclonal antibody conjugation

Hi Danai,

Polyclonals conjugate just like anything else....there's nothing stopping the chemistry. In fact, Fluidigm/SBio even sells a couple in their product line, like IgA/Product Number 3148007B.

The formal disadvantage with a polyclonal product is that it's made of more than one antibody, with varying properties and varying specificities (precise target epitopes, etc). And, will potentially vary more lot to lot than a monoclonal prep.

But there's no reason not to at least give it a try.


Mike
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DanaiBD

Participant

Posts: 7

Joined: Mon Oct 24, 2022 12:28 pm

Post Tue Oct 25, 2022 3:23 pm

Re: Polyclonal antibody conjugation

Thank you very much :D

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